Intraspecific Genetic Variation in the Japanese Loach (Misgurnus anguillicaudatus) Revealed by Random Amplified Polymorphic DNA (RAPD) Analysis
نویسندگان
چکیده
منابع مشابه
Random Amplified Polymorphic DNA (RAPD) Markers
Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplificatio...
متن کاملAnalysis of Different Ploidy and Parent–Offspring Genomic DNA Methylation in the Loach Misgurnus anguillicaudatus
In this study, we selected natural polyploidy loach (diploid, triploid and tetraploid) and hybrid F₁ generation obverse cross (4 × 2) and inverse cross (2 × 4) by diploids and tetraploids as the research model. The MSAP (methylation-sensitive amplified polymorphism) reaction system was established by our laboratory to explore methylation levels and pattern diversification features at the whole ...
متن کاملRandom amplified polymorphic DNA (RAPD) is a PCR based technique for identifying genetic variation
Introduction The quality of DNA, obtained by various protocols and subjected to various storage conditions can be judged by comparing the PCR amplification profiles. The most commonly used technique that can be accessed by almost any laboratory is RAPD . Random amplified polymorphic DNA (RAPD) is a PCR based technique for identifying genetic variation. It involves the use of a single arbitrary ...
متن کاملIntraspecific differentiation of Hancornia speciosa revealed by simple sequence repeat and random amplified polymorphic DNA markers.
Hancornia speciosa, popularly known as mangabeira, is a fruit tree native to the Brazilian Cerrado that shows great economic potential, due to its multiple uses. Intraspecific classification of this species is difficult because it shows high morphological diversity. An early study of the species reported that there are six botanic varieties that differ morphologically mainly in the shapes of th...
متن کاملUtility of Random Amplified Polymorphic DNA (RAPD) In Forensic Entomology
Forensic entomology is the study of insects found around the crime scene and are helpful for the estimation of time elapsed since death of a person i.e., post mortem interval (PMI). There are number techniques available for the identification of insect species and Random Amplified Polymorphic DNA (RAPD) is one of those. RAPD is a useful technique designed for the detection of sequence polymorph...
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ژورنال
عنوان ژورنال: Pakistan Journal of Biological Sciences
سال: 2001
ISSN: 1028-8880
DOI: 10.3923/pjbs.2001.877.880